PCR#:	LY0002		5/22/13
	25 μL reaction
		# Tubes:	13
MasterMix	Single Tube (μL)	Total in MM (μL)
EconoTaq MM	12.5	162.5
H20	2.5	32.5
FW	3	39
RV	3	39
DNA	4	X
TOTAL:	25
FW Primer	THYF
RV Primer	THYR
Tube#	Sample
1	223167
2	582151
3	20568
4	588022
5	104610
6	110278
7	108297
8	15575
9	588479	hardly any DNA--need to extract more
10	113765	hardly any DNA--need to extract more
11	control
Program:	Omar1
Annealing Temp:	50	longer annealing time (:45)

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PCR#:	LY0003		5/22/13
	25 μL reaction
		# Tubes:	13
MasterMix	Single Tube (μL)	Total in MM (μL)
EconoTaq MM	12.5	162.5
H20	2.5	32.5
FW	3	39
RV	3	39
DNA	4	X
TOTAL:	25
FW Primer	STAT5A F
RV Primer	STAT5A R
Tube#	Sample
1	223167
2	582151
3	20568
4	588022	need to extract more DNA
5	104610	need to extract more DNA
6	110278
7	108297
8	15575
9	588479	hardly any DNA--need to extract more
10	113765	hardly any DNA--need to extract more
11	control
Program:	touchdowntchup
Annealing Temp:	54