PCR#:	LY0002		5/22/13
	25 μL reaction
		# Tubes:	13
MasterMix	Single Tube (μL)	Total in MM (μL)
EconoTaq MM	12.5	162.5
H20	2.5	32.5
FW	3	39
RV	3	39
DNA	4	X
TOTAL:	25
FW Primer	THYF
RV Primer	THYR
Tube#	Sample
1	223167
2	582151
3	20568
4	588022
5	104610
6	110278
7	108297
8	15575
9	588479	hardly any DNA--need to extract more
10	113765	hardly any DNA--need to extract more
11	control
Program:	Omar1
Annealing Temp:	50	longer annealing time (:45)

-----------------------------------------------------------------------------------

PCR#:	LY0003		5/22/13
	25 μL reaction
		# Tubes:	13
MasterMix	Single Tube (μL)	Total in MM (μL)
EconoTaq MM	12.5	162.5
H20	2.5	32.5
FW	3	39
RV	3	39
DNA	4	X
TOTAL:	25
FW Primer	STAT5A F
RV Primer	STAT5A R
Tube#	Sample
1	223167
2	582151
3	20568
4	588022	need to extract more DNA
5	104610	need to extract more DNA
6	110278
7	108297
8	15575
9	588479	hardly any DNA--need to extract more
10	113765	hardly any DNA--need to extract more
11	control
Program:	touchdowntchup
Annealing Temp:	54

First PCR as a PhD student. Sigh.

PCR#:	LY0001
	30 μL reaction
		# Tubes:	8
MasterMix	Single Tube (μL)	Total in MM (μL)
Buffer	3	24
MgCl2	3	24
dNTPs	0.5	4
Taq	0.5	4
H20	13	104
FW	3	24
RV	3	24
DNA	4	X
FW Primer	THYF
RV Primer	THYR
Tube#	Sample
1	588460
2	580656
3	110278
4	108297
5	560781
6	control
Program:	Omar1
Annealing Temp:	50

Sample #s correlate with AMCC AMNH tissue number.