First PCR as a PhD student. Sigh.
Sample #s correlate with AMCC AMNH tissue number.
2_19_THY_LO.jpg)
| PCR#: | LY0001 | ||
| 30 μL reaction | |||
| # Tubes: | 8 | ||
| MasterMix | Single Tube (μL) | Total in MM (μL) | |
| Buffer | 3 | 24 | |
| MgCl2 | 3 | 24 | |
| dNTPs | 0.5 | 4 | |
| Taq | 0.5 | 4 | |
| H20 | 13 | 104 | |
| FW | 3 | 24 | |
| RV | 3 | 24 | |
| DNA | 4 | X | |
| FW Primer | THYF | ||
| RV Primer | THYR | ||
| Tube# | Sample | ||
| 1 | 588460 | ||
| 2 | 580656 | ||
| 3 | 110278 | ||
| 4 | 108297 | ||
| 5 | 560781 | ||
| 6 | control | ||
| Program: | Omar1 | ||
| Annealing Temp: | 50 |
Sample #s correlate with AMCC AMNH tissue number.
2_19_THY_LO.jpg)
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